OLYMPUS DP2-BSW DOWNLOAD FREE

Untreated spleen cells were used as an additional negative control. View at Google Scholar D. The images were analyzed with Image-Pro Plus 6. The serum samples were diluted 1: Descriptive metadata is automatically attached to every image and a user-defined sample name and descriptions may also be added.

Uploader:	Doulmaran
Date Added:	23 August 2014
File Size:	20.41 Mb
Operating Systems:	Windows NT/2000/XP/2003/2003/7/8/10 MacOS 10/X
Downloads:	89741
Price:	Free* [*Free Regsitration Required]

Histology The salivary glands and lacrimal glands were obtained from the mice sacrificed in each group four weeks after the second immunization. PBS immunizations triangle were used olympuw an additional control.

The muscarinic acetylcholine 3 receptor M3R plays a key role in mediating exocrine protein secretion in the salivary and lacrimal glands.

Immunization with the M3R — peptide induced peptide-specific antibodies. The spleen cells were obtained from the mice sacrificed in each group four weeks after the second immunization. FireWire connectivity makes the camera easy to set up and use in the laboratory.

Archived Products

Immunofluorescence staining was performed as described previously with some modifications [ 3 ]. The lymphocytic infiltration in the salivary glands and lacrimal glands in M3R — treated mice compared to control peptide and PBS was measured four weeks after the second immunization.

However, few studies have been performed on the M3R — peptide of the second extracellular loop. These results showed that peptide-specific antibodies were successfully and specifically generated only in animals immunized with the M3R — peptide.

Archived Products > DP25 Digital Camera

Sera were collected from mice in each group before immunization light gray barand four weeks following the second immunization with M3R — black barCP charcoal gray baror PBS white barand the samples were diluted 1: These include live shading correction, brightness and contrast adjustments, and pseudo-coloring. Antibody Titer Determination The sera were collected every seven days after the second immunization. Red blood cells were removed by treatment with a 0.

Here, we assessed the cytokine profiles of cell culture supernatants after spleen klympus were cocultured with either the M3R — peptide, CP, or PBS. Four image resolutions are available: Injections of PBS were used as an additional control. Antigen-specific regulatory cells may suppress autoimmune response by inhibiting the generation of Th-1, Th-2, and Th cells.

Journal of Immunology Research

Lin Yang performed most of the experiments. Then, we evaluated the Th-2 cytokines IL-4 and IL and found that the activity of IL-4 was significantly decreased in the serum and cell culture supernatants of M3R — immunized mice, although decreases in IL were not observed.

Lin Yang, Guoan Yang, and Yongfu Wang conceived the study and participated in the design and in the interpretation of results. No significant decrease in IL secretion was observed.

Olympus LM - Center for Microscopy and Imaging

Untreated spleen cells served as an additional control light gray bar. The results showed that the secretion of IL in cell culture supernatant decreased following immunization with M3R — as compared to the control groups. Materials and Methods 2. As immunization with M3R — peptide conjugated to an immunogenic carrier protein such as KLH may induce some uncertain immunological responses and immunization with the entire second extracellular loop tends to be pathogenic and, in addition, Naito et al.

Moreover, recent data have shown that the levels of TGF- mRNA transcripts and protein were significantly higher in animals with an experimental model of dry eye [ 15 ].

No financial or other relationships could lead to a conflict of interests. However, no changes in the concentration of IL were observed in the current study, suggesting that IL may not be regulated following immunization with M3R — View at Google Scholar D. Please send a copy of the enquiry to my email address.

All authors read and approved the final paper. Annotation, simple interactive measurements, post-acquisition image enhancement tools and other features provide even more functionality. However, no trend toward decreased concentrations of IL was observed Figure 2 b. Leukocytic infiltrations were analyzed with Image-Pro Plus olympuss. The lymphocytic infiltration histological score was graded using the method proposed by Greenspan et al.